Journal: Bio-protocol

Article Title: Rapid Multiplexed Reduced Representation Bisulfite Sequencing Library Prep (rRRBS)

doi: 10.21769/BioProtoc.3171

Figure Lengend Snippet: Preparation of adapter ligation mix

Article Snippet: If non-methylated adapters are used, the bisulfite treatment will convert non-methylated cytosine into thymidine, and this will interfere with the recognition of the adapter sequence in future reactions ( ). table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Reagent Volume for one reaction DNase/RNase free water 8.5 μl T4 DNA ligase enzyme 10x buffer 5 μl NEB methylated adapters 10 μl T4 DNA ligase (2,000,000 U/ml) 1.5 μl Open in a separate window caption a8 Preparation of adapter ligation mix On ice, add 25 μl of the master mix to each 26 μl DNA sample.

Techniques: Ligation, Methylation

Journal: Bio-protocol

Article Title: Rapid Multiplexed Reduced Representation Bisulfite Sequencing Library Prep (rRRBS)

doi: 10.21769/BioProtoc.3171

Figure Lengend Snippet: Preparation of qPCR mix for library amplification

Article Snippet: If non-methylated adapters are used, the bisulfite treatment will convert non-methylated cytosine into thymidine, and this will interfere with the recognition of the adapter sequence in future reactions ( ). table ft1 table-wrap mode="anchored" t5 Table 3. caption a7 Reagent Volume for one reaction DNase/RNase free water 8.5 μl T4 DNA ligase enzyme 10x buffer 5 μl NEB methylated adapters 10 μl T4 DNA ligase (2,000,000 U/ml) 1.5 μl Open in a separate window caption a8 Preparation of adapter ligation mix On ice, add 25 μl of the master mix to each 26 μl DNA sample.

Techniques: SYBR Green Assay